药用植物大黄种子基因组DNA的提取方法研究

摘要

通过传统CTAB法、改良CTAB法、高盐低pH法、改良SDS法、试剂盒法等5种方法,对大黄种子进行基因组DNA提取,为了筛选一种适合大黄种子基因组DNA的提取方法,同时用紫外分光光度法和琼脂糖凝胶电泳对5种方法所提的DNA进行检测,并将5种方法所提取的DNA进行PCR扩增检测.结果表明,高盐低pH法提取的DNA得率最好,时间较短,价格便宜,PCR扩增条带清晰,能满足分子标记的要求.%This paper aimed to find out a suitable method for genomic DNA extraction of Rheum L.seeds from Tibet,used medicinal plant the traditional CTAB method,improved CTAB method,high salt and low pH method,the improved SDS method,reagent kit.In addition,at the same time the process was by ultraviolet spectrophotometry and agarose gel electrophoresis for five kinds of methods of DNA testing,and five methods by DNA PCR amplification detection,according to the results of PCR detection,high salt and low pH method to extract DNA test three parallel PCR amplification.The results showed that the high salt and low pH method to extract was fast,convenience of operation,the extracted DNA quality was better,PCR amplification of the stripe was clear,which can satisfy the requirement of molecular markers.