Automated high throughput purification of genomic DNA from plant leaf or seed using MagneSil~(~R) paramagnetic particles

摘要

Three different methods of automated high throughput purification of genomic DNA from plant materials processed in 96 well plates are described One method uses MagneSil~(~R) paramagnetic particles to purify DNA present in single leaf punch samples or small seed samples, using 320ul capacity 96 well plates which minimizes reagent and plate costs. A second method uses 2.2 ml and 1.2 ml capacity plates and allows the purification of larger amounts of DNA from 5-6 punches of materials or larger amounts of seeds. The third method uses the MagneSil~(~R) ONE purification system to purify a fixed amount of DNA, thus simplifying the processing of downstream applications by normalizing the amounts of DNA so they do not require quantitation. Protocols for the purification of a fixed yield of DNA, e.g. 1 ug, from plant leaf or seed samples using MagneSil~(~R) paramagnetic particles and a Beckman-Coulter BioMek~(~R) FX robot are described. DNA from all three methods is suitable for applications such as PCR, RAPD, STR, READIT~(~R) SNP analysis, and multiplexed PCR systems. The MagneSil~(~R) ONE system is also suitable for use with SNP detection systems such as Third Wave Technology's Invader~(~R) methods.