HPV16 E2对宫颈癌SiHa细胞上皮间质转化的影响

摘要

Objective To investigate the influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa cells.Methods Lipofectamine was used to transfect p-CAG-MYC-HPV16 E2 gene into cervical cancer SiHa cells.The morphology of cells was observed before and after transfection with phase-contrast microscope,and the expression of Twist2,E-cadherin and Vimentin before and after transfection was determined by RT-PCR and Western blotting.Results HPV16 E2 was successfully transfected into SiHa cells.After transfection,the epithelial cells became spindle-shaped,with enlarged intercellular space,and the expression of Twist2 and Vimentin was up-regulated,while the expression of Ecadherin was down-regulated.Conclusion HPV16 E2 can induce epithelial-mesenchymal transition of cervical cancer cells,and promote cervical carcinogenesis.%目的 探讨HPV16 E2对宫颈癌SiHa细胞上皮间充质细胞转化的影响.方法 采用脂质体转染p-CAG-MYC-HPV16 E2至宫颈癌SiHa细胞中,倒置相差显微镜下观察转染前后细胞形态的改变;RT-PCR及Western blotting检测转染前后Twist2、上皮细胞标志物E-cadherin及间质细胞标志物Vimentin表达的变化.结果 成功转染HPV16 E2至宫颈癌SiHa细胞中.转染后,上皮细胞由椭圆形变成梭形,细胞间隙增大;Twist2和Vimentin表达上调,E-cadherin表达下调.结论 HPV16 E2能在体外诱导宫颈癌细胞发生上皮间充质细胞转化,促进早期癌变.