甜瓜果实八氢番茄红素脱氢酶基因（CmPDS）的克隆与表达

摘要

A full-length cDNA encoding Phytoene desaturase(PDS), named as CmPDS (GenBank:KC507802), was isolated from the fruit of‘M01-3’of Muskmelon(Cucumis melo L.) by RT-PCR and RACE. The cDNA has an open reading frame of 1731 bp and encodes a polypeptide of 576 amino acids. Sequence analysis showed that deduced CmPDS protein was highly homologous to other PDS proteins from different species, especially with Cucurbita moschata, Momordica charantia and Ricinus communis, that the identity of amino acid sequence was up to 92.6%~87.8%. Real time PCR analysis indicated that CmPDS expressed in different organs. The transcription levels of CmPDS in leaves and fruit of 25 d after pollination were higher than that in stems, roots and tubers respectively. With the development of fruit, the expression of the gene rised but reduced in the end.%以甜瓜自交系‘M01-3’果实cDNA为模板，采用RT-PCR和RACE技术，克隆了甜瓜果实八氢番茄红素脱氢酶（PDS）基因cDNA全长序列，命名为CmPDS（基因注册号：KC507802）。序列分析表明，该基因有开放阅读框1731 bp，编码576个氨基酸，与其他植物PDS氨基酸序列具有较高的同源性，尤其与黄瓜、南瓜、苦瓜PDS氨基酸序列高达92.6%~87.8%。荧光定量分析表明，该基因在甜瓜不同器官中均有表达，在叶片和授粉后25 d的果实中表达量较高，在根中表达量最低；随果实发育成熟，该基因表达量呈现先升高后降低趋势。