菌株Eurotium sp.JW043的分离及培养基碳氮源筛选

摘要

茯茶是黑茶精加工的一种,具有消食健胃、降血压、降血脂等功效.在茯茶制备工艺过程中,通过人工接种"发酵剂",可明显提高茯茶的"发花"效率、缩短"发花"周期.从陕西泾阳茯茶中分离出一株"金花菌",具有开发为茯茶"发酵剂"的潜质.结合形态学和分子生物学方法确定其为散囊菌属真菌,命名为 Eurotium sp.JW043,并对适于其菌丝生长和产孢的培养基条件进行优化.通过单因素实验筛选出蔗糖和酵母浸膏分别作为碳源和氮源.进一步通过正交试验探讨最适合该菌株菌丝生长和产孢的培养基组成.结果表明,适于该菌株孢子产生的培养基组成为每100mL培养基中含蔗糖5.0g、酵母浸膏1.5g、硫酸镁0.5g和磷酸氢二钾0.1g;适于该菌株菌丝生长的培养基为每100mL培养基中含有蔗糖7.5g、酵母浸膏1.5g、硫酸镁0.5 g和磷酸氢二钾0.1 g.对进一步开发菌株 Eurotium sp.JW043为茯茶新型发酵剂,进而提高茯茶"发花"效率具有一定的应用价值.%Fu brick tea is a kind of dark tea with post fermentation.It has the functions of di-gestion,lower blood pressure and blood fat.In the procession of making Fu brick tea,artifi-cial inoculation of "fermenting agent" can increase the "flowering" efficiency significantly.The"flowering" period would also be shorten.In current study,a strain of Eurotium sp.,which has the potential to be developed as a"fermentative agent"for Fu brick tea,was isola-ted from Fu brick tea produced in Jingyang county,Shaanxi province.Combined with mor-phological and molecular biological methods,it was identified as a fungus of the genus Euro-tium sp.,named Eurotium sp.JW043,and the conditions of the medium suitable for its myce-lial growth and sporulation were optimized.Single factor experiments were used to screen su-crose and yeast extracts as carbon and nitrogen sources,respectively.Further orthogonal ex-periments were conducted to determine the composition of the medium that was most suit-able for the mycelial growth and sporulation of the strain.The results showed that the com-position of the medium suitable for spore production of this strain was sucrose 5.0 g,yeast extract 1.5 g,magnesium sulfate 0.5 g,and dipotassium hydrogen phosphate 0.1 g per 100 mL of medium.The culture medium contained 7.5 g of sucrose,1.5 g of yeast extract,0.5 g of magnesium sulfate,and 0.1 g of dipotassium hydrogen phosphate per 100 mL of the medi-um was suitable for mycelial growth of thestrain..This study has a certain application value for the further development of the strain Eurotium sp.JW043 as a new fermenting agent for Fu brick tea.