大蒜素对人食管癌EC-109细胞形态结构的影响

摘要

Study to human esophageal cancer EC-109 cells as the research object,explore Allicin on morphological structure of EC-109 cells.The inhibitory ratio of cells was measured by trypan blue stain assay.By inverted microscope,fluorescence microscope to observe different concentrations of garlic to act on human esophageal cancer EC-109 cell morphological changes.The result shown that Allicin can inhibit proliferation of human esophagus cancer cell line EC-109,and induce apoptosis of EC-109 cells,and in a range of time,dose-dependent,the IC50 is (28±1.54)μg/mL.Allicin can make EC-109 cells DNA fragmentation.%　　以人食管癌EC-109细胞为研究对象，探讨大蒜素对人食管癌EC-109细胞生长抑制及对形态结构的影响．采用台盼蓝拒染法测定人食管癌EC-109细胞生长抑制率，通过倒置显微镜、荧光显微镜观察不同质量分数的大蒜素作用于人食管癌EC-109细胞的形态变化．结果表明，大蒜素能抑制人食管癌 EC-109细胞增殖，诱导 EC-109细胞凋亡，在一定范围呈时间、剂量依赖性，作用48 h的IC50为（28±1.54）μg/mL．经大蒜素诱导后，人食管癌EC-109细胞形态结构出现典型的凋亡特征．