苦瓜多糖对人食管癌EC-109细胞形态结构的影响

摘要

Study to human esophageal cancer EC-109 as the research object,explore purification MCP2 of momordica charantia polysaccharide on morphological structure of human esophageal cancer EC-109.The inhibitory ratio of cells was measured by trypan blue stain assay.By inverted microscope to observe different concentrations of purification MCP2 of momordica charantia polysaccharide to act on human esophageal cancer EC-109 cell morphological changes.The result shown that purification MCP2 of momordica charantia polysaccharide can inhibit proliferation of human esophageal cancer EC-109,and induce apoptosis of human esophageal cancer EC-109,and in a range of time,dose-dependent,the IC50 is (285.45±36.64)μg/mL.Purification MCP2 of Momordica charantia polysaccharide can make human esophageal cancer EC-109 DNA fragmentation.%以人食管癌EC-109细胞为研究对象，探讨苦瓜多糖组分MCP2对人食管癌EC-109细胞生长的抑制，以及对其形态、结构的影响．采用台盼蓝拒染法测定人食管癌EC-109细胞生长抑制曲线，通过倒置显微镜观察不同质量分数的苦瓜多糖组分MCP2作用于人食管癌EC-109细胞导致的形态变化．结果表明，苦瓜多糖组分MCP2能抑制人食管癌EC-109细胞增殖，诱导人食管癌EC-109细胞凋亡，在一定范围内呈时间、剂量依赖性，作用48 h的IC50为（285.45±36.64）μg/mL．经苦瓜多糖组分MCP2诱导后，人食管癌EC-109细胞形态结构出现典型的凋亡特征．