目的:比较人血清透明质酸(HA)夹心化学发光免疫分析和竞争化学发光免疫分析,对两种方法的相关性、线性范围和检测效果进行评价.方法:分别应用人血清HA夹心和竞争化学发光免疫分析对197例血清样本进行HA含量检测,并对检测结果进行分析,同时对两种方法的精密度、回收率和灵敏度进行比较.结果:夹心法的准确性和精密度都要高于竞争法,在95%可信限下其检测灵敏度能达到0.66 μg/L,而竞争法只能达到7.26 μg/L,两种方法均能准确测定血清中HA含量,其相关性良好,线性关系为y=1.1893x-25.974(r=0.9871),两组数据之间没有显著性差异(t=1.966,P>0.05).结论:两种方法都能准确测量人血清中HA含量,但夹心化学发光免疫分析的灵敏度、重复性、回收率都优于竞争法.%Objective To evaluate the correlation, linear range and detection effect of sandwich and competitive chenulumines-cence immunoassay for the detection of hyaluronic acid from human serum the performance of the 2 methods was compared. Methods The level of hyaluronic acid in 197 sera was detected by the sandwich and competitive chemiluminescence immunoassay, and the results were analyzed statistically. The precision, recovery and sensitivity of the 2 methods were assessed simueltaneously. Results The accuracy and precision of sandwich chemiluminescence immunoassay were better than competitive chemiluminescence immunoassay. The detection limit of sandwich chemiluminescence immunoassay could leach 0.66 μg/L and the competitive chemiluminescence im-munoassay just could reach 7.26 μg/L. However, both methods could exactly detect hyaluronic acid from serum. The correlation of them was y = 1.1893χ-25.974(r =0.9871), whose results showed no significant difierence(t = 1.966,P =0.664). Conclusion Sandwich and competitive chemiluminescence immunoassay could all exactly detecting hyaluronic acid concentration of serum, but sensitivity, repeatability and recovery of sandwich method were better than those of competitive method's.
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