化学发光免疫分析检测人血清中的癌胚抗原

摘要

Based on the catalyzed oxidation of horseradish peroxidase (HRP) to the hydrogen peroxide and 3-(4-hydroxyphenyl propionate) (PHPPA) reaction, the fluorescent product, 3-(4-hydroxyphenyl propionate) dimer, can react with bis(2,4, 6-trichlorophenyl)oxalate(TCPO) and hydrogen peroxide to enhance chemical luminescence by the participation of imidazole enhancer in acetonitrile medium. With horseradish peroxidase(HRP) labeled Carcinoembryonic Antigen(CEA) monoclonal antibody, by “sandwich type” CEA immune response, a simple, sensitive, and rapid chemiluminescence immunoassay method for the determination of Carcinoembryonic Antigen (CEA) in human serum samples has been developed. There was a very good linear correlation between response and amount of CEA in the range of 1.0-80.0 μg/L (r=0. 9989). The detection limit was 0. 3 μg/L (S/N=3). The relative standard deviation (n=11) was 3.9％. The proposed method has been used for the determination of CEA in human serum.%基于辣根过氧化物酶(HRP)催化H2O2氧化3-(4-羟苯基)丙酸(PHPPA),生成能发荧光的3-(4-羟苯基)丙酸二聚体.在乙腈介质中,在增强剂眯唑参与下,与双[2,4,6-三氯苯基]草酸酯(TCPO)和H2O2反应产生强化学发光.用辣根过氧化物酶(HRP)标记癌胚抗原(CEA)单克隆抗体,通过CEA的双抗夹心免疫反应,建立了简单、灵敏和快速检测人血清中的癌胚抗原(CEA)含量的化学发光免疫分析方法.CEA在1.0～80.0 μg/L范围具有良好的线性关系;检出限为0.3 μg/L(S/N=3),相对标准偏差(n=11)为3.9%.已成功用于人血清样品中CEA含量的测定.