Objective To investigate clinical diagnostic value of collagen contents of hepatic fibrosis tissue and serum growth factors level in patients with chronic viral hepatitis B and correlate with hepatic lesion. MethOds 80 patients with chronic viral hepatitis B were divided into 3 groups(more light,n = 31, moderate, n= 20, severe n=29) , 34 healthy people served as control group. Colla-gen contents of hepatic tissue microsection were determined by colorimetry, serum level of TGF-β1 was determined by RIA, CTGF, PDGF, TIMP-1 and MMP-9 levels were measured by enzyme RIA . The data was processed and analyzed. Results The contents of collagen of hepatic tissue were significant higher in all patients than those in the controls ( P ＜ 0.05, P ＜ 0.01) , the changes also showed significant difference between the contents of two groups of patients(P＜0.05, P＜0.01). In more light patients, the serum levels of TIMP-1 and MMP-9 were no more significant difference than those in controls (all P ＞ 0.5), but the levels were significant higher in both groups of moderate and severe patients(P＜0.05 ,P ＜0.01) , the changes also significant difference between the contents of two groups of patients (P＜0.05, P＜0.01). The serum levels of CTGF and PDGF were all significant higher in more light patients than those in the controls (all P ＜0.05) , while the serum levels of TGF-β1 were not significant difference in more light patients than those in the controls (P ＞0.5). In moderate and severe patients,the levels of three markers were all significant higher than those in the controls (a11 P＜0.01) , the changes also significant difference between the contents of two groups of patients(P＜ 0.05, P ＜0.01). Conclusion The changes of 6 laboratory items were closely related with development and progression of chronic viral hepatitis B and determination of which would be useful fo realize the development of hepatopathy and pathogenesis.%目的:探讨慢性乙型肝炎患者肝组织胶原含量(gepatic collagen content)、血清结缔组织生长因子(connective tissue growth factor,CTGF)、血小板衍生生长因子(platelet-derived growth factor,PDGF)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)及基质金属蛋白酶组织抑制因子-1(tissue inhibitor of MMP-1,TIMP-1)水平的变化、临床诊断价值以及与肝损害程度的关系.方法:本文将80例慢性乙型肝炎患者按不同的病情分为轻度组(31例),中度组(20例)和重度组 (29例)三组进行研究.设体检健康人34名作为正常对照组.肝组织胶原含量采用切片比色法测定;血清TGF-β1水平采用RIA;CTGF、PDGF、TIMP-1及MMP-9水平均用ELISA.并将测定结果进行统计学分析.结果:患者肝组织切片胶原含量轻度组显著高于正常对照组(P0.05)外,中及重度两组水平的变化同本文胶原含量.CTGF、PDGF和TGF-β1三项生长因子水平则轻度组前两项与正常对照组比较有显著性升高(P0.05);中度及重度组三项指标均较正常对照组升高非常显著(P均<0.01).三组患者间两两比较均呈递增性升高,差异均较显著(P<0.05,P<0.01).本文取重度组的胶原含量与两组酶类数据和CTGF、PDGF和TGF-β1三项生长因子水平进行了相关性分析,结果均呈显著正相关(r=0.572,P<0.01;r=0.612,P<0.01);(r=0.612,P<0.01;r=0.545,P<0.01;r=0.611,P<0.01).结论:六项实验室项目水平的变化与肝损害的程度关系密切,这些测定对于了解肝病的进展和病理机制有一定帮助.
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