VEGF-A和 Ki67在膀胱移行细胞癌中的表达及临床意义

摘要

目的：观察血管内皮生长因子A（VEGF-A）和细胞核增殖抗原（Ki67）在膀胱移行细胞癌中的表达，研究VEGF-A与膀胱移行细胞癌中淋巴管密度（ LVD）和微血管密度（ MVD）是否有关。方法采用免疫组织化学方法观察VEGF-A及Ki67在60例膀胱移行细胞癌组织中的表达，用D2-40标记淋巴管， CD34标记微血管，进行淋巴管和微血管的计数。结果 VEGF-A阳性组的LVD和MVD与阴性组的LVD和MVD比较均有显著性差异（P＜0．05）。淋巴结转移组的LVD和MVD与未转移组的LVD和MVD比较均有显著性差异（P＜0．05）。 Ki67阳性表达率与淋巴结转移有关（P＜0．05）。结论在膀胱移行细胞癌的淋巴管生成和微血管生成中VEGF-A发挥重要作用。 Ki67可作为膀胱移行细胞癌转移趋势的有效指标。%Objective To research the relevance between vascular endothelial growth factor A ( VEGF-A) and lymphatic vessel density ( LVD) and microvessel density ( MVD) as well as the role of VEGF-A in lymphangiogenesis and angiogenesis in bladder transitional cell carcinoma , the expression of VEGF-A and Ki67 were measured .Methods VEGF-A and Ki67 expression were studied by immunostain in bladder transitional cell carcinoma .Lymphatic vessels and microvessels were detected by D 2-40 and CD34 respectively .Results Higher LVD and MVD were shown in VEGF-A positive group than in VEGF-A negative group respectively (P<0.05). LVD and MVD were significantly higher in lymph node metastatic group than in nonmetastatic group (P<0.05). Ki67 expression was closely associated with lymph node metastasis in bladder transitional cell carcinoma respectively (P<0.05).Conclusions VEGF-A plays an important role in lympangiogenesis and angiogenesis in bladder transitional cell carcinoma .Ki-67 can be used to predict the metastatic potential of bladder transitional cell carcinoma .