双酚A(BPA)对小鼠睾丸发育的影响

摘要

双酚A (BPA)是一种人工合成的添加剂,主要用于聚碳酸酯塑料和环氧树脂的强化,所以含有BPA的工业产品在我们的生活中随处可见.同时,BPA作为一种环境类雌激素,又是一种对包括人类在内的哺乳动物内分泌系统的干扰物.本研究的目的就是探讨BPA对小鼠睾丸发育的危害作用,以及对精液品质的影响.结果表明:在小鼠睾丸发育的过程中,BPA能够导致睾丸发育不正常,具体表现在导致曲精细管的发育异常；BPA处理7周之后对小鼠睾丸产生不可逆转的损害,并且这种损害直接影响曲精细管的直径与小鼠睾丸的生精作用,并且导致小鼠精液中精子数量的下降.然而,BPA对于精细胞的Igf2r等印迹基因的DNA甲基化进程没有影响.此外,BPA连续处理7周后的小鼠的生长发育以及生育能力都受到严重影响.综上所述,BPA能够导致小鼠睾丸发育过程异常,导致小鼠精液中精子的数量的下降,但不影响Igf2r等印迹基因的DNA甲基化.%Bisphenol A (BPA), a high production volume chemical used in a wide variety of consumer products, is ubiquitous in our daily life. Meanwhile, it is a contaminant interfering with endocrine system of mammals including humans. The aim of this study is to explore the effect of BPA on the spermatogenesis and provide the evidence of BPA against semen quality. Our results have demonstrated that BPA could affect the development of testis by damaging the seminiferous tubule. BPA can lead to irreversible damage to testis and the decline of sperm in semen of mice. However, BPA has no effect on the methylation of imprinted gene Igf2r. We also confirm BPA having the influence of the male mouse after 7 weeks treatment. In conclusion, BPA accelerates declines the spermatogenesis and leads to abnormal testis development in mice without interference on DNA methylation of imprinted genes such as Igf2r.