双酚A通过雌激素受体影响小鼠睾丸和附睾兰尼碱受体基因的表达

摘要

Objective:To explore the possible effects of bisphenol A on ryanodine receptors (RyRs) in mouse testis and epididymis,the role of the two ERs was also investigated.Methods:Adult male wild type (WT),ERs knock out mice (αERKO and βERKO) were randomly divided into control and bisphenol A [100 μg/(kg · d) for 30 days via intragastric administration] treatment groups.Quantitative real time PCR was used to examine RyR1,RyR2 and RyR3 genes of the testis and epididymis.Results:The mRNA expression of RyR1 and RyR3 were increased in bisphenol A-treated animals,however,RyR2 was evidently decreased.In the epididymis,the mRNA expression of RyR1,RyR2 and RyR3 were significantly increased (P ＜ 0.05).Bisphenol A up-regulation of RyR1 of the epididymis mRNA expression was dependent on ERα,whereas bisphenol A regulation of RyR2 of the testis and RyR2,RyR3 of the epididymis appeared to be dependent on both ERα and ERβ.Conclusion:The ERs signalling was necessary for the regulation of the RyR channels mRNA expression by bisphenol A,which could affect the male reproductive function.%目的:观察双酚A对雄性小鼠睾丸和附睾中兰尼碱受体(ryanodine receptors,RyRs)基因表达的影响及雌激素受体(estrogen receptor,ERα和ERβ)在其中的作用.方法:将野生型小鼠、ERα基因敲除小鼠(αERKO)和ERβ基因敲除小鼠(βERKO)均随机分为双酚A组[100 μg/(kg·d)]和对照组,经口灌胃染毒30 d后(对照组以玉米油灌胃),荧光定量PCR法检测睾丸和附睾中RyR1,RyR2和RyR3 mRNA表达的变化.结果:双酚A可使小鼠睾丸中RyR1和RyR3 mRNA表达水平均明显升高,而RyR2 mRNA表达水平显著降低,附睾中RyR1、RyR2和RyR3 mRNA表达水平明显升高(P均＜0.05).应用ERKO小鼠实验结果显示,双酚A通过ERα和ERβ影响睾丸RyR2以及附睾RyR2和RyR3 mRNA的表达,对附睾RyR1 mRNA的调控则依赖于ERα.结论:双酚A可以影响雄性小鼠睾丸和附睾中兰尼碱受体3种亚型的基因表达,FRα和ERβ在此过程中发挥了重要作用.