利用ARTP诱变育种技术选育白色金针菇突变菌株

摘要

本研究首先从市售工厂化栽培的五株白色金针菇菌株中筛选出一株品质优良的菌株AR0作为本次诱变育种的出发菌株.然后利用常压室温等离子体(ARTP)诱变育种技术选育出6株白色金针菇突变株.通过对突变株与出发菌株的抗病性、纤维含量测定及亲缘关系鉴定,结果表明突变株AR12和AR17为抗病性强、纤维含量低的目标新菌株.其优良品质表现如下:突变菌株AR12与出发菌株AR0相比,菌丝体抗P.tolaasii能力提高15.49％,菌丝体纤维含量降低15.82％;突变株AR17与出发菌株AR0相比,菌丝体抗P.tolaasii能力提高1.90％,菌丝体纤维含量降低36.31％.%In this study,a superior strain (AR0) was selected from five white Flammulina velutipes which were attained from the industrialized cultivation.Then,AR0 was utilized as the original strain to breeding six kinds of white Flammulina velutipes using Atmospheric and Room Temperature Plasma (ARTP) induced mutation.Afterwards,the disease resistance,cellulose content of the original and mutant strain were analyzed and their genetic relationship were identified too.The results have shown that,AR 12 and AR 17 were the target strain which have higher disease resistance and lower cellulose content properties.Compared with the original strain (AR0),the P.tolaasii resistivity,cellulose content of mutant stain (AR 12) were improved 15.49 ％ and reduced 15.82 ％,respectively,and for mutant strain (AR 17),the P.tolaasii resistivity,cellulose content were improved 1.90 ％ and reduced 36.31％.