以小麦再生苗叶片为外植体的愈伤组织诱导再生培养体系建立

摘要

By using the embyogenic callus as the explants, the effects of subculture time, plant growth regulators and leaf size on the callus induction and regeneration of leaf tissues of wheat plantlets were studied. Experimental results showed that embryogenic callus with a subculture time of 1 - 6 months had a higher regeneration frequency. In addition, it was found that leaf explants from leave with hight of 4 cm and cuhutred on a MS medium with the plant hormones of 2,4 - D 2mg/L and NAA lmg/L gave the highest callus induction frequency. Therefore, a callus induction and regeneration system from leaf tissues of wheat plantlets was established.%以小麦幼胚愈伤组织为外植体，研究继代培养时间、外源激素及叶片大小对小麦试管苗叶片愈伤组织诱导和再生的影响。结果表明：继代培养1～6个月的胚性愈伤组织，有较高的再生苗频率；当生长素浓度为2，4-D2mg／L＋NAA1mg／L、叶片高度为4cm时，小麦试管苗叶片愈伤组织的诱导率最高，从而建立了小麦试管苗叶片愈伤组织诱导和再生的培养体系。