白藜芦醇对血管紧张素Ⅱ诱导心肌肥大的抑制作用

摘要

Objective:To investigate the inhibitory effects of resveratrol on Ang II -induced cardiac hy-pertrophy in H9C2 cells,so as to elucidate the role of ERK1 /2 -CREB signaling pathway during cardiac hy-pertrophy.Methods:Ang II was applied to establish the cardiacmyocytes hypertrophy model of H9C2 cells. The Cell surface area of H9C2 cells was measured by Image Pro Plus 6.1 software.The total protein content of H9C2 cells was detected by BCA method.The protein expression of CREB and p -CREB were determined by western blot.Results:Compared with the control group,cell surface area was increased significantly in Ang II group (P <0.01).The total protein content of the cells was also increased significantly in Ang II group (P <0.01).The total protein content of Resv group (0.01μM,0.1μM,1μM +Ang II )was decreased in a concentration -dependent manner.Ang II stimulated phosphorylation of CREB.Resv (0.01μM,0. 1μM,1μM +Ang II group)could reduce the expression level of p -CREB in a concentration -dependent manner.Conclusion:Resv could inhibit AngII induced cardiac hypertrophy,and its mechanism might be through inhibiting the increases of protein content,surface area and the expression of CREB.%目的：研究白藜芦醇（Resveratrol Resv）对血管紧张素 II （Angiotensin II，AngII）诱导H9C2细胞肥大的抑制作用，并探讨其机制。方法：通过建立 Ang II 诱导 H9C2细胞肥大模型，采用Image Pro Plus 6．1软件测量细胞表面积大小；BCA 法测定细胞总蛋白含量，Western －blot 方法检测H9C2细胞 p －CREB 和 CREB 蛋白的表达。结果：与对照组相比，AngII （0．1μM）孵育后，细胞表面积增加，细胞蛋白含量增加了46％（P ＜0．01）。Resv （0．01μM ＋AngII 组，0．1μM ＋AngII 组，1μM ＋AngII 组）能浓度依赖地减少蛋白含量。Ang II 诱导 H9C2细胞 p －CREB 蛋白的表达升高。不同剂量 Resv 对 AngII 诱导 H9C2细胞 p －CREB 蛋白的表达均有抑制作用，并且呈浓度依赖。结论：Resv 抑制 AngII 介导的 H9C2细胞肥厚，其机制可能是通过抑制细胞表面积增大、细胞蛋白含量增多和 CREB 蛋白表达实现的。