硬腭骨膜牵张成骨过程中ACTA和FS的表达研究

摘要

Objective:To study the mRNA expression of activin A( ACTA) and follistatin( FS) at different stages in canine with cleft palate ( CP) during periosteal distraction osteogenesis ( PDO). Methods; Experimental CP models were established in 15 juvenile dogs by surgery. 2 months after the surgery distraction device was placed under the periosteum at the edge of CP with a force of (2. 45 ± 0.20) N. Every 3 dogs were sacrificed 10, 20, 30, 40 and 60 days respectively after the placement of distraction device, bone samples were harvested, RNA was extracted from the newly formed bone tissue of the distracted side and the control side respectively. ACT A and FS expressions were detected by RT-PCR. Results; A small amount of ACT A and FS mRNA were detected in the bone tissue samples of the control side from 10 to 60 days( P > 0. 05 ) . Higher expression of ACT A and FS mRNA was observed in the samples of the 10th day in the distracted side. ACT A mRNA increased by 1. 78 , 2. 82, 4. 27 and 3. 04 times respectively 20, 30, 40 and 60 days after the fixation of the distraction device; FS mRNA increased by 1.52, 2. 12, 2.47 and 1.90 times. Conclusion; ACT A and FS play important role in regulating osteogenesis in periosteum distraction.%目的:检测犬腭裂骨膜牵张成骨各时期ACT A和FS mRNA的表达,探讨ACT A/FS系统对其修复的调节模式.方法:用幼犬15只建立腭裂模型,2月后,于腭裂裂隙缘骨膜下安放牵张器,牵张力(2.45±0.20)N.牵张器放置后10、20、30、40和60 d,各处死3只,取牵张侧及对照侧骨组织,提取RNA并用RT- PCR检测ACT A和FS mRNA的表达.结果:对照侧10～60 d标本检出少量ACT A和FS mRNA,各组间比较无统计学意义(P＞0.05).牵张侧10 d标本可检出ACT A和FS mRNA；20、30、40及60 d ACT A mRNA含量分别为10 d的1.78、2.82、4.27及3.04倍,FS mRNA分别为1.52、2.12、2.47及1.90倍.结论:在牵张期间,ACT A可能直接或间接地增强膜内成骨,FS参与ACT A生物活性的调节.