莱菔硫烷对涎腺腺样囊性癌细胞系ACC-M增殖和凋亡的影响

摘要

Objective:To study the effects of sulforaphane(SFN)on the proliferation and apoptosis of salivary adenoid cystic carci-noma ACC-Mcells in vitro.Methods:ACC-Mcells were treated with SFN at the doses(μmol/L)of 5,10,20,30,40,60,80 and 100 for 24,48 and 72 hours,respectively.The growth inhibition was examined with MTT assay and typan blue exclusion assay.Morpholo-gy of ACC-M cells was observed with phase contrast microscope,giemsa staining and transmission electron microscope.Flow cytome-try with Annexin-V-FITC/PI double staining was used to detect the apoptosis of ACC-M cells.Results:SFN inhibited the prolifera-tion of ACC-Mcells,the IC50 values(μmol/L)after 24,48 and 72 h treatment were 75.6,21.3 and 16.5 respectively.The highest inhibition rate was 89.2%.The growth inhibition rate of SFN on ACC-Mcells was positively correlated with concentrations of SFN and treatment time.SFN induced the apoptosis of ACC-Mcells in a dose and time dependent manner(P<0.01).Conclusion:SFN can inhibit proliferation and induce apoptosis of salivary adenoid cystic carcinoma ACC-M cells time and dose-dependently.%目的：研究莱菔硫烷（SFN）对涎腺腺样囊性ACC-M癌细胞增殖和凋亡的影响。方法：分别用终浓度为5、10、20、30、40、60、80、100μmol／L的SFN处理ACC-M细胞24、48、72 h。用MTT比色试验和台盼蓝拒染实验检测细胞的增殖和存活情况；倒置显微镜、Giemsa染色、透射电镜观察细胞形态学变化；Annexin-V-FITC和PI双标记流式细胞仪检测细胞凋亡情况。结果：SFN对ACC-M有明显的增殖抑制作用，最高抑制率达89．2％，作用24、48、72 h时的IC50（μmol／L）分别是75．6、21．3、16．5，增殖抑制率与药物浓度和作用时间具有明显的正相关性。SFN可诱导ACC-M细胞凋亡，凋亡率随处理时间延长和药物浓度增加而上升（P＜0．01）。结论：SFN具有抑制涎腺腺样囊性癌细胞系ACC-M增殖和诱导其凋亡的作用，且具有浓度和时间依赖性。