类胰蛋白酶和TI M-3双阳性肥大细胞在人不同程度牙周炎组织中的量化研究

摘要

Objective:To quantify the tryptase and T cell immunoglobulin mucin domain-3(TIM-3)double positive mast cells in hu-man chronic periodontitis tissue using double immunofluorescence staining.Methods:25 healthy controls,28 chronic mild periodontitis and 30 chronic advanced periodontitis patients were included.The gingival specimens were stained with HE for histology,and with double immunofluorescence staining for the identification of tryptase and TIM-3 double positive mast cells in gingival tissue.Results:In chronic periodontitis tissue the degree of gingival inflammation was significantly increased,the densities(cells/mm2 )of tryptase and TIM-3 double positive mast cells were significantly increased(P<0.05),in addition,that in chronic advanced periodontitis group was significantly higher than in the chronic mild periodontitis group(P<0.05).Conclusion:Tryptase and TIM-3 double positive mast cells has the similar tendency as the severity of periodontitis inflammation in human periodontitis tissue.Tryptase and TIM-3 double positive mast cells may play an important role in human chronic periodontitis.%目的：观察类胰蛋白酶（tryptase）和T细胞免疫球蛋白粘蛋白-3（TIM-3）在慢性牙周炎牙龈组织肥大细胞中的表达。方法：纳入正常对照受试者25例，轻度牙周炎患者28例，重度牙周炎患者30例。牙龈组织标本经HE染色，光学显微镜下观察；免疫荧光双染色，荧光显微镜下观察。结果：牙龈组织中的炎症反应程度与慢性牙周炎的病变程度趋势一致。牙周炎组牙龈组织tryptase和TIM-3双阳性肥大细胞数量均显著升高（P＜0．05）；重度牙周炎组牙龈组织中的双阳性肥大细胞数量显著高于轻度牙周炎组（P＜0．05）。结论：慢性牙周炎牙龈组织中的tryptase和TIM-3双阳性肥大细胞数量与牙周炎病变程度的趋势相一致，提示tryptase和TIM-3双阳性肥大细胞可能参与慢性牙周炎的免疫调节。