柠檬精油抗氧化活性的研究

摘要

Objective:To explore the antioxidant activity of natural plant ingredients lemon essential oil(LEO),limonene(LIM) and tea polyphenol(TP).Methods:The UV-Vis spectrometry was used to determine the 3 agents on the scavenging activity of 1 ,1 diphenyl-2-trinitrobenzene hydrazine free radicals(DPPH·)and hydroxyl free radical (·OH).Results:LEO,LIM and TP showed scavenging effect on the 2 kinds of free radicals.The (DPPH·)scavenging effect:LEO(IC50 =0.01 4)>TP(IC50 =0.01 4)>LIM (IC50 =0.002);the (·OH)scavenging effect:TP(IC50 =0.079)>LEO(IC50 =0.01 3)>LIM(IC50 =0.004).In a certain con-centration range,scavenging effect was increased with the concentration increase.Conclusion:As a nontoxic natural extract,lemon essential oil has strong antioxidant activity.%目的：探究天然植物成分柠檬精油、柠檬烯及茶多酚对自由基的清除能力。方法：采用 UV-Vis 光谱测定法测定3组药物对1，1－二苯基－2－三硝基苯肼自由基（DPPH·）和羟基自由基（·OH）的清除作用。结果：柠檬精油、柠檬烯及茶多酚对2种自由基都有较好的清除作用，对（DPPH·）的清除效果：柠檬精油（IC50＝0．014）＞茶多酚（IC50＝0．014）＞柠檬烯（IC50＝0．002）；对（·OH）清除效果：茶多酚（IC50＝0．079）＞柠檬精油（IC50＝0．013）＞柠檬烯（IC50＝0．004）。在一定浓度范围内，清除率随浓度的增大而增大。结论：柠檬精油作为无毒的天然提取物，具有较强的抗氧化活性。