浓缩生长因子提取液对 MC3T3-E1细胞影响的实验研究

摘要

Objective:To evaluate the effect of concentrate growth factor extract(CGFe)on the proliferation and differentiation of MC3T3-E1 osteoblasts.Methods:MC3T3-1 cells were cultured with and without CGFe respectively.At 1 ,3,5 d of culture the pro-liferation of the cells was detected by MTT assay,the activity of alkaline phosphatase (ALP)of the cells was examined by ALP kit;at 1 4,21 d of culture the calcium nodus formation was observed by Alizarin red staining;RUNX2 and OSX expression was quantified by real-time PCR.Results:With the extension of culture time,the proliferation,ALP activity,the calcium nodus and the level of RUNX2 and OSX mRNA of the experimental group were increased more than those of the control group.Conclusion:CGFe may pro-mote the proliferation and differentiation of osteoblasts.%目的：探讨浓缩生长因子提取液（CGFe）对 MC3T3-E1细胞增殖、分化的影响。方法：实验组和对照组分别使用含有及不含有 CGFe 的α-MEM培养液培养 MC3T3-E1细胞。培养1、3、5 d 后 MTT 法测定细胞增殖，ALP 试剂盒检测碱性磷酸酶活性；培养7、14 d 后茜素红染色观察钙结节形成；荧光实时定量 PCR 测定 RUNX2和 OSX mRNA 的表达。结果：随着培养时间延长，实验组的细胞增殖、碱性磷酸酶活性、钙结节数目及基因表达量均高于对照组（P ＜0．05）。结论：浓缩生长因子提取液能有效地促进 MC3T3-E1细胞的增殖、分化。