The effects of basic fibroblast growth factor or epidermal growth factor on equine tendon fibroblasts and dermal fibroblasts in vitro.

摘要

Scope and method of study. The purpose of this study was to examine the biological effects of varying concentrations (0, 2, 5, 10, and 20 ng/ml) of basic fibroblast growth factor (FGF) or epidermal growth factor (EGF) on four biological parameters of fibroblasts involved in wound healing of the equine tendon and skin of the extremities: proliferation, migration, collagen type I and type III synthesis. Tendon and dermal fibroblasts extracted from a total of six horses between the ages of 5–8 years were utilized for in vitro procedures. A commercial assay was used for proliferative indices and results were evaluated with a t-test. Chemotactic chambers were used for migration assays, and monlayer ELISA's were used for pro-collagen type I and III synthesis. Results of these 3 assays were evaluated with a t-test, and linear regression for dose dependent changes.; Findings and conclusions. Neither basic fibroblast growth factor nor epidermal growth factor enhanced the proliferative indices of equine tendon or dermal fibroblasts, Basic fibroblast growth factor and epidermal growth factor, individually, significantly increased chemotactic activity, in a dose dependent fashion, for both equine tendon and dermal fibroblasts. Basic fibroblast growth factor was shown to significantly increase synthesis of type I and type III synthesis, in a dose dependent manner, in equine tendon and dermal fibroblasts. Dermal fibroblasts were more responsive to basic fibroblast growth factor, at a lower dose and with a higher level of pro-collagen synthesis, than tendon fibroblasts. Epidermal growth factor was shown to significantly decrease type I and type III pro-collagen synthesis, in a dose dependent manner, in equine tendon and dermal fibroblasts. Dermal fibroblasts responded to epidermal growth factor, at a lower dose and at a greater decrease in pro-collagen synthesis, than equine tendon fibroblasts.; Within the context of wound healing, epidermal growth factor would not be a good candidate for enhancing the processes of wound healing. However, basic fibroblast growth factor may enhance wound healing in both equine tendons and skin of the extremities through enhanced chemotaxis of and pro-collagen type I synthesis by fibroblasts, two biological parameters necessary for proper wound healing to occur.