IFN-γ+874 A/T位点和IL-2-330 T/G位点的单核苷酸多态性与复发性口腔溃疡易感性的关系

摘要

目的：：研究IFN-γ+874A/T位点与IL-2-330T/G位点的单核苷酸多态性( SNP)与复发性口腔溃疡( RAU)易感性的关系。方法：采用ELISA方法检测138例RAU组患者和124例正常对照组中IFN-γ及IL-2的血清含量,并用SSP-PCR和RFLP-PCR对全血进行IFN-γ+874位点与IL-2-330位点的单核苷酸多态性的检测分析。结果：RAU组患者血清中IFN-γ及IL-2水平均显著低于正常对照组(P<0.05)。 IFN-γ+874位点在基因型频率与等位基因频率的分布2组间差异有显著性(P<0.05)。 IFN-γ+874位点基因型AA的OR值=9.964为易感基因型,A等位基因为易感等位基因, OR值=3.801。 IL-2-330位点在基因型频率与等位基因频率的分布2组间差异无显著性(P>0.05)。 IFN-γ+874位点中,携带A等位基因患者患复发性口腔溃疡的风险是携带T等位基因者的3.801倍。结论：IFN-γ+874位点中携带A的等位基因与RAU易感性相关,IL-2-330位点与RAU的易感性无关。%Objective:To explore the possible relationship between recurrent aphthous ulcer( RAU) and the single nucleotide polymor-phism(SNP)of IFN-γ+874A/T site and IL-2-330T/G site. Methods:138 RAU patients and the 124 health control subjects were in-cluded. IFN-γand IL-2 serum concentration were detected by ELISA. IFN-γ+874A/T site and IL-2-330T/G site of the whole blood were detected by RFLP-PCR and SSP-PCR. Results:IFN-γand IL-2 levels in serum of the patients were significantly lower than those of the controls(P<0. 05). There was significant difference in the genotype frequencies and allele frequencies of IFN-γ+874A/T site between the 2 groups(P<0. 05). The AA genotype(OR=9. 964)and the A allele (OR=3. 801) at the IFN-γ+874A/T site was with high-risk of RAU. Carry A allele in patients with risk of RAU was to carry the T allele of 3. 801 times. There was no significant differ-ence in the genotype frequencies or allele frequencies of IL-2-330T/G site between the 2 groups(P>0. 05). Conclusion: IFN-γ+874A/T site was associated with the risk of RAU. But,IL-2-330T/G site was not.