中晚期宫颈癌DNA-PKcs表达与放化疗敏感性的相关性研究

摘要

目的:探讨中晚期宫颈癌组织DNA依赖性蛋白激酶(DNA-PKcs)的表达与放化疗敏感性的关系,寻找预测宫颈癌放化疗敏感性的指标.方法:用免疫组化法对42例ⅡB期～ⅢB期宫颈癌患者治疗前及治疗开始21天宫颈癌活检标本进行DNA-PKcs表达检测,分析DNA-PKcs在放化疗前后的表达及变化情况与病理缓解、肿瘤消退时间的相关性.结果:高度病理缓解组患者放化疗前DNA-PKcs的表达强度明显低于低度病理缓解组,放化疗敏感性与DNA-PKcs的表达强度呈负相关(Z=-5.583,r=-0.513,P=0.000,).放化疗后DNA-PKcs表达下降的程度与肿瘤消退一半的时间呈负相关(r=-0.924,P=O.01).结论:DNA-PKcs的表达能够预测宫颈癌放化疗敏感性；放化疗早期宫颈癌组织DNA-PKcs的表达下降,提示治疗效果好.%Objective;To investigate the correlation of the expression of DNA-PKcs in cervical cancer stage JIB - IJjB with the sensitivity of chemoradiotherapy . Methods:The expression of DNA-PKcs in 42 specimens of cervical carcinoma was detected by immunohistochemistry before treatment and in 21 days during treatment. The correlation of the expression DNA-PKcs with the histologial response and regression of tumor was analyzed. Results:The expression of DNA-PKcs was much lower in highly histologial responding group than that in the low responding group, the sensitivity of chemoradiation was negatively correlated with the expression of DNA-PKcs(Z= -5. 583,r= -0. 513,P=0. 000). The degree of decreased expression of DNA-PKcs after chemoradiation was negatively correlated with the time of 50% tumor volume regression (r= -0. 924,P=0. 01). Conclusions;The expression of DNA-PKcs can predict the sensitivity of chemoradiation in cervical cancer. The reduced expression of DNA-PKcs during chemoradiation in cervical cancer gives a hint of good prognosis.