Objective:To investigate the expression and significance of Activin A in maternal and cord serum idiopathic fetal growth restriction (IFGR), B-cell lymphoma associated x protein (Bax), and X-linked inhibitor of apoptosis protein (XIAP) in placenta of idiopathic fetal growth restriction (IFGR). Methods:From July 2010 to May 2011, 30 pregnant women with IFGR underwent cesarean section in our hospital were selected as experimental group, and 30 normal term pregnant women underwent cesarean section due to social factors were matched as control group. Activin A in maternal and umbilical cord serum were analyzed by enzyme-linked immunosorbent assay (ELISA), while Bax and XIAP in placenta were detected by immunohis-tochemical method. Results:The level of Activin A both in maternal and cord serum of the experimental group (102. 659 ±16.467, 57.752 ±13.498) ng/ml ,were significantly higher than those in control group (75. 927 ±10. 519,29. 870 ±5. 992) ng/ml(P<0.05). The expression of Bax in placental syncytiotropho-blast in experimental group (146. 513 ± 10. 611) was significantly increased as compared to the control group (113. 672 ±9. 631) (P <0. 05). The expression of XIAP in placental syncytiotrophoblast in experimental group (114. 562 ±5.167) was significantly decreased as compared to the control group (144. 430 ± 7. 311) (P <0. 05). The level of Activin A in maternal and cord serum was positively correlated with the expression of Bax in placenta in experimental group (p<0. 05), whilst it showed negative correlation with the expression of XIAP in placenta in experimental group (P<0. 05). Moreover, the expression of XIAP in syn-cytiotrophoblast was found negetively correlated with Bax (P<0. 05). Conclusions:The increased Activin A in maternal and cord serum, as well as the increased expression of Bax and decreased expression of XIAP in placenta, may play important roles in the pathogenisis of IFGR. The increased Activin A may lead to excessive apoptosis of placental syncytiotrophoblast, which could impact the development and function of placenta and impede the fetal growth, by means of up-regulating expression of Bax and down-regulating expression of XIAP in IFGR.%目的:探讨特发性胎儿生长受限(IFGR)患者母血和脐血激活素A(Activin A)及胎盘组织中B细胞淋巴瘤因子相关x蛋白(Bax)和X-连锁凋亡抑制蛋白(XIAP)的表达及意义.方法:选取剖宫产分娩的IFGR孕妇30例作为实验组,同期因社会因素剖宫产分娩的正常足月孕妇30例作为对照组.采用双抗体夹心酶联免疫吸附法( ELISA)测定两组母血、脐血Activin A 水平;免疫组化法检测两组胎盘组织中Bax与XIAP的表达.结果:实验组母血、脐血Activin A 水平( 102.659±16.467,57.752±13.498) ng/ml均明显高于对照组(75.927±10.519,29.870±5.992) ng/ml(P＜0.05).Bax、XIAP在胎盘合体滋养细胞中的表达,实验组Bax 平均灰度值( 146.513±10.611)明显高于对照组(113.672±9.631),差异有统计学意义(P＜0.05)；实验组XIAP平均灰度值(114.562±5.167)明显低于对照组(144.430±7.311),差异有统计学意义(P＜0.05).实验组母血、脐血中Activin A水平与胎盘组织中Bax表达均呈正相关(P＜0.05),与胎盘组织中XIAP表达均呈负相关(P＜0.05)；实验组胎盘组织中Bax表达与XIAP表达呈负相关(P＜0.05).结论:母血、脐血中Activin A升高及胎盘组织中Bax表达增加、XIAP表达降低可能是IFGR发病的重要环节之一.在IFGR中Activin A可能通过上调Bax的表达、下调XIAP的表达,促进胎盘滋养细胞过度凋亡,影响胎盘的发育和功能,进而影响胎儿生长发育.
展开▼
