Objective To observe the changes of the concentrations of TNF-a and IL-8 in bronchoalveolar lavage fluid (BALF) in rabbits after long time one-lung ventilation. Methods Thirty-five New Zealand white rabbits were divided into control group (Group C, n = 5) , two-lung ventilation group (Group TLV,n = 15) , and one-lung ventilation group (Group OLV ,n = 15). According to the time of ventilation , Group TLV and OLV were then divided into 3 sub-groups (1 h group, 3 h group, and 5 h group, each n = 5). Group C received experimental operation but without ventilation. Heart rate (HR), median blood pressure (MAP), the value of blood gas analysis, and the concentrations of TNF-a and IL-8 in BALF were measured. Results As compared with the basic value , HR and PaC02 were increased, while MAP, pH, PaO2, and SaO2 were decreased in 5 h group of Group OLV (P < 0.01). But there were no significant changes of HR , MAP, pH and PaCO2 in Group TLV (P>0.05). Concentrations of TNF-a and IL-8 in left and right lung were increased in 5 h group of Group OLV (P < 0.01) ; those in left lung were increased in 5 h group of Group TLV (P < 0.01) ; and those in left lung in 5 h group of Group OLV were significantly different from those in left lung in 5 h group of Group TLV (P < 0.01). Conclusion Lung injury in one-lung ventilation is severer than that in two-lung ventilation , and was proportional to the time of ventilation.%目的:通过兔单肺通气模型,观察长时间单肺通气后肺灌洗液中TNF-α、IL-8浓度的变化.方法:选择新西兰大白兔35只,分为对照组(C组)、双肺通气组(TLV组)和单肺通气组(OLV组),每组5只兔.TLV组和OLV组按通气时间1、3、5 h分为3亚组,随后恢复双肺通气30min.对照组只行实验操作而不通气.观察通气时的心率(HR)、平均血压(MAP)、血气分析值、支气管肺泡灌洗液(BALF)中TNF-α、IL-8浓度.结果:与通气开始相比,OLV组在通气5hHR增高、MAP降低(P<0.01),pH、PaO2、SaO2在单肺通气后降低,PaCO2在OLV5h后升高(P<0.01);TLV组HR、MAP、pH、PaCO2在各观察时点无统计学差异(P>0.05).OLV组左右肺的TNF-α和IL-8值在通气5 h增高(P<0.01);TLV组的左肺的TNF-α和IL-8值在通气5 h增高(P<0.01);OLV组通气5 h左肺TNF-α、IL-8值与TLV组左肺比较有显著差异(P<0.01).结论:单肺通气时肺损伤程度与单肺通气时间成正比,单肺通气肺损伤比双肺通气肺损伤严重.
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