Objective To investigate the clinical significance of testing serum kisspeptin in central precocious puberty (CPP) girls. Methods Sixty eight CPP girls and 68 healthy girls was studied from December 2012 to December 2014. HEK293 cells were cultured. Luciferase reporter assay was performed to verify the binding of miR-137 to the 3′UTR of KISS1. Serum miR-137 level was levaluated by qRT-PCR. Level of serum luteinizing hormone , prolactin , follicle stimulating hormone , thyrotropin , free thyroxine and estradiol was evaluated by chemi-luminescence immunoassay. The level of serum kisspeptin was detected by ELISA. Results MiR-137 was confirmed to bind to the 3′UTR of KISS1. The level of serum miR-137 was downregulated and kisspeptin was enhanced in CPP girls. The expression of miR-137 and kisspeptin was negatively correlated. Serum miR-137 level was negatively related to bone age and bone age advancement. According to the results of GnRH stimulating test, serum miR-137 was related to peak LH and peak/basal LH ratio. Conclusions MiR-137 could bind to the 3′UTR of KISS1. MiR-137 may be a potential biomarker for CPP assisted diagnosis.%目的：评价检测中枢性性早熟（CPP）女童血清miR-137的临床意义。方法：收集2012年12月至2014年12月在我院门诊就诊的68例 CPP 女童及68例健康女童血清；培养 HEK293细胞，荧光素酶活性分析验证miR-137与KISS1结合情况；qRT-PCR 检测miR-137的表达；化学发光法检测血清黄体生成素、泌乳素、卵泡刺激素、促甲状腺素、游离甲状腺素、雌二醇水平；酶联免疫吸附试验检测血清吻素的表达。结果：miR-137与KISS13′UTR 结合；CPP女童血清中miR-137降低，吻素水平升高，两者呈负相关；miR-137与骨龄、骨龄年龄差呈负相关；GnRH 激发试验中，miR-137与LH 峰值、峰值／基础 LH 比值呈负相关。结论：miR-137可与KISS13′UTR 结合，可作为CPP辅助诊断的潜在生物学标志物。
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