目的：探讨10-羟基-2-癸烯酸（10-HDA）联合L-抗坏血酸2-葡糖苷（AA-2G）对长波紫外线（UVA）诱导的成纤维细胞光损伤的保护作用。方法：将原代培养人皮肤成纤维细胞分为空白对照组、UVA照射组、10-HDA＋AA-2G组。通过细胞增殖活性测定、细胞衰老β-半乳糖苷酶（SA-β-gal）染色、活性氧（ROS）检测观察10-HDA＋AA-2G对UVA诱导的细胞毒性、细胞衰老状态及活性氧簇（ROS）水平的影响。结果：与空白对照组相比，UVA照射组的细胞增殖活性明显下降（P ＜0．05），SA-β-gal阳性细胞的数量和ROS水平均显著上升（均P ＜0．01）。与UVA照射组相比，10-HDA＋AA-2G组的细胞增殖活性显著上升（P ＜0．01），SA-β-gal染色阳性细胞和ROS水平明显下降（分别为P ＜0．05和P ＜0．01）。结论：10-HDA＋AA-2G可以抑制UVA诱导的细胞毒性、细胞衰老和ROS升高，提示其对UVA诱导的成纤维细胞光损伤具有保护作用，可能成为一种防治皮肤光老化的有效组合。%Objective To explore the protective effects of 10-HDA combined with AA-2G on UVA-induced photodamage in fibroblasts. Methods The primary cultured human skin fibroblasts were divided into three groups: blank control group, UVA irradiation group and 10-HDA+AA-2G group. The cell viability and cellular senescent state were analyzed using CCK-8 and senescence associated-β-galactosidase (SA-β-gal) staining, respectively. Fluorometric assays were performed to detect the formation of reactive oxygen species (ROS) in the cells. Results Compared with those in control group, the cell viability was decreased (P < 0.05), and the SA-β-gal positive cells and ROS level were significantly increased (P<0.01 for both) in UVA-irradiated group. Compared with those in UVA-irradiated group, the cell viability was significantly increased (P < 0.01), and the SA-β-gal positive cells and ROS level were significantly reduced (P < 0.05, P < 0.01, respectively) in the 10-HDA combined with AA-2G group. Conclusion Fibroblasts treated with 10-HDA and AA-2G are significantly protected from UVA-induced cytotoxicity, cellular senescence and ROS, indicating that 10-HDA combined with AA-2G has photoprotective effects. Therefore, 10-HDA combined with AA-2G may be a potential combination for the prevention and treatment of skin photoaging.
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