CGTase ascorbic acid-2-glucoside AA-2G The method for producing ascorbic acid-2-glucosideAA-2G using the immobilization of recombinant cyclodextrin glucanotransferase CGTase

摘要

The present invention constructs a recombinant expression vector capable of mass expression of recombinant CGTase by utilizing the genetic recombination technology of the existing AA-2G sugar transfer enzyme CGTase, transforms it into E. coli, and expresses various expression conditions that can be overexpressed. It provides a method of optimizing, extracting and purifying the recombinant CGTase in large quantities. In addition, the produced CGTase binds to a specific resin through an enzyme immobilization operation, and provides a method for manufacturing AA-2G, a vitamin C derivative, by utilizing this.