Objective To evaluate the condition of oxidative stress and immunosuppression in early stage of severe sepsis,and investigate the correlation between them. Methods A prospective random control study in-cluded patients group(n=51)and control group(n=31). The concentration of serum superoxide dismutase was measured by enzyme linked immunosorbent assay(ELISA),CD4+CD25+Treg% was measured by flow cytometry , respectively. The difference between two groups was compared and the correlation between parameters in patients group was evaluated. Results The concentration of serum SOD was lower than control group (P < 0.01). CD4+CD25+Treg% significantly high,compared to the control group(P < 0.01). There was no strong correlation be-tween parameters in patients group. Conclusion Oxidative stress and immunosuppression are exist in the early stage of severe sepsis.%目的 观察严重脓毒症患者早期氧化应激和免疫抑制状态,并探讨其相关性.方法 前瞻性随机对照研究,设立病例组(n=51)和对照组(n=31),分别采用ELISA法测定血清超氧化物歧化酶(SOD)浓度,流式细胞技术测定外周血CD4+CD25+Treg%,比较两组之间的差异,探讨病例组各参数间的相关性.结果 病例组血清SOD浓度[36.0(15.0,50.9)ng/mL]显著低于对照组[60.3(40.2,88.2)ng/mL](P<0.01),而CD4+CD25+Treg%则显著高于对照组[6.49(4.5,11.63)vs.5.36(3.92,6.39),P<0.01],提示病例组存在显著的氧化应激和免疫抑制.病例组血清SOD浓度和CD4+CD25+Treg%未发现显著相关性.结论 严重脓毒症患者早期存在免疫抑制和氧化应激.
展开▼
