目的：分析188例血清乙型肝炎病毒外膜大蛋白（LHBs）检测结果，探讨LHBs的临床应用价值及其与乙型肝炎病毒DNA（HBV-DNA）、乙型肝炎病毒e抗原（HBeAg）之间的相关性。方法用酶联免疫吸附试验（ELISA）法定量检测乙型肝炎病毒标志物、LHBs，采用实时荧光聚合酶链反应（PCR）定量检测HBV-DNA。结果在相同的乙型肝炎病毒标志物模式中，HBV-DNA与LHBs检测结果的阳性率相关（r=0.891，P=0.001）。HBeAg阳性患者血清的检测结果显示，LHBs测定值与HBeAg测定值相关（r=0.267，P=0.019）；乙型肝炎病毒标志物阴性及其余乙型肝炎病毒标志物模式LHBs测定值与HBeAg、HBV-DNA测定值均不相关（P>0.05）。结论 LHBs与HBV-DNA阳性率具有一致性，能够反映乙型肝炎患者体内病毒复制情况，可以视作HBeAg阴性患者中病毒复制的一个新的血清学指标，临床应用中应将LHBs与HBsAg及HBV-DNA联合检测。%Objective To explore the value of clinical application of (hepatitis B virus large surface protein, LHBs) and the correlation between LHBs and HBV-DNA, or LHBs and hepatitis B virus e antigen(HBeAg) by analyzing 188 cases of serum LHBs. Methods HBV markers and LHBs were quantified by using enzyme linked immune-sorbent assay (ELISA) method, HBV-DNA were quantified by the real-time fluorescence polymerase chain reaction (PCR). Results In the same hepatitis B virus markers pattern, the positive rates of HBV-DNA and LHBs were correlated (r=0.891) and the difference was statistically significant (P=0.001). LHBs was only correlated to HBeAg in patients with HBeAg positive (r=0.267,P=0.019). No significant correlation were found between LHBs and HBeAg or HBV-DNA in patients with all HBV markers negative pattern (P>0.05). Conclusion The positive rate of LHBs was consistent with HBV-DNA, which may reflect the virus replication in patients with HBV infection. It can be regarded as a new serologic indicator of virus replication in patients with HBeAg negative and should be detected combined with HBsAg and HBV-DNA.
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