内毒素在酒精性肝病肠损伤中的作用

摘要

Objective To study the effect of endotoxin on intestinal mucous injury in rats with alcohol-induced liver disease. Methods 15 patients with alcoholic liver disease and 15 healthy persons were included in this study .The serum endotoxin was detected by ELISA,and serum MDA was detected by spectrophotometric method. Twenty c57/BL mice were randomly divided into model and control group (n=10 each). The control group was fed with Lieber-Decarli liquid without ethanol,and mice in the model group were fed with 4% ethanol of Lieber-Decarli liquid. The serum endotoxin was examined after 10 weeks,and the intestinal and liver tissues were assessed with HE staining. Results The serum endotoxin in alcoholic liver disease group and control group were 0.52±0.54 Eu/L and 0.47±0.34Eu/(P<0.05),respectively;The serum MDA in alcoholic liver disease group and control group were 5.6±5.0nmolAnl and 3.7±3.4nmol/ml(P>0.05), respectively;The serum endotoxin in the model rats and control rats were 0.38±0.05 Eu/L and 0.13±0.02 Eu/L(P<0.01), respectively;The histopathological scores of colon injuries in the model group was much higher than that in control group (10.3±1.3vs 4.8±1.2,P=0.0l). Conclusion Alcoholic liver disease is always complicated by intestinal mucous injury. Endotoxemia and intestinal mucosal injury has certain correlation in alcoholic liver disease.%目的 探讨内毒素在酒精性肝病肠损伤中的作用.方法 选择酒精性肝病患者15例和健康不饮酒者15例.采用ELISA法测定血清内毒素;采用分光光度法测定血清丙二醛.另将20只小鼠随机分为对照组和模型组,每组10只,分别喂饲Lieber-Decarli无酒精和含酒精液体饲料.10周后处死小鼠,检测血清内毒素,并进行肝脏和肠组织形态学观察.结果 酒精性肝病组和对照组血清内毒素水平分别为0.52±0.54 Eu/L和0.47 ±0.34 Eu/L(P<0.05);酒精性肝病组和对照组血清丙二醛水平分别为5.6±5.0nmol/ml和3.7±3.4nmol/ml(P>0.05);模型组和对照组小鼠血清内毒素水平分别为0.38±0.05 Eu/L和0.13±0.02 Eu/L(P<0.01);模型组肝细胞明显脂肪变,结肠组织损伤病理学评分为10.3 ±1.3分,较对照组明显升高(4.8±1.2分,P=0.01).结论 酒精性肝病伴发了肠粘膜损伤,内毒素可能参与了发病过程.