基于酶与金-普鲁士蓝磁性纳米复合物修饰电极的制备及其对H2 O2的响应

摘要

A H2 O2 selective electrode has been presented. The gold-prussian blue magnetic nanocomposite ( Au-PB )was firstly electrochemically deposited onto the magnetic carbon paste electrode( MCPE )by cyclic voltammetry method,and then horseradish peroxidase( HRP)was self-assembled noto the Au-PB preparing MCPE electrode through the Au -NH -bond. Under optimal conditions. The resulting selective electrode dis-played a rapid response to H2 O2 and the linear range of the modified electrode was from 3. 53 × 10 -6 ~3. 53 × 10 -3 M( correlation coefficient r=0 . 998 6 )with a lower detection limit of 1 . 96 × 10 -6 M and a sensitivity of 42. 28 mV/decade. Furthermore,the modified electrode exhibited fast response speed and low cost.%利用磁场作用将金-普鲁士蓝磁性纳米复合物（ Au-PB）固定于磁性碳糊电极（ MCPE）表面，进而通过金-氨键组装辣根过氧化物酶（ HRP），根据Au-PB与HRP共同催化还原H2 O2作用，制备了一种用于过氧化氢检测的修饰电极（MCPE/Au-PB/HRP）.利用循环伏安法（CV）和电化学交流阻抗谱（EIS）对电极的修饰过程进行了表征，初步研究了MCPE/Au-PB/HRP对不同浓度H2 O2的响应性能，其检测灵敏度为42.28 mV/dec-ade，线性响应范围为3.53×10-6~3.53×10-3 M，相关系数为0.9986，检测下限为1.96×10-6 M.所制备的修饰电极响应迅速、灵敏度高、检测范围较宽，适用于微量H2 O2的快速检测，为有机磷农药传感器的制备奠定了基础.