绿原酸对佐剂性关节炎模型大鼠抗炎作用及机制研究

摘要

目的：探讨绿原酸对佐剂性关节炎模型动物抗炎作用及机制。方法采用大鼠足跖注射 Freund's 完全佐剂形成佐剂性关节炎（AA），随机分为空白对照组、模型组、阳性对照组（尼美舒利）、绿原酸低剂量组（25 mg· kg -1）、中剂量组（50 mg·kg -1）和高剂量组（100 mg·kg -1）。观察绿原酸对佐剂性关节炎模型大鼠超敏反应与左右足跖宽度、血清肿瘤坏死因子（TNF -α）、白细胞介素-2（IL -2）、循环免疫复合物（CIC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH - Px）和丙二醛（MDA）的含量。结果绿原酸中剂量组（50 mg·kg -1）、高剂量组（100 mg·kg -1）能够降低含量，升高 SOD 和 GSH - Px 水平，比较差异有统计学意义（P ﹤0.05）。结论绿原酸具有抗佐剂性关节炎（AA）的作用，并通过降低 TNF -α、IL -2、CIC 和 MDA 含量及提高抗氧化能力发挥作用。%Objective To investigate the effects of the anti - inflammatory effect of chlorogenic acid on adjuvant arthri-tis rats. Methods Treated with various concentrations of chlorogenic acid on adjuvant arthritis(AA),then divided into six groups as control group,model group,positive control group(nimesulide),low dose(25 mg·kg - 1 ),medium dose(50 mg· kg - 1 )and high dose(100 mg·kg - 1 )of chlorogenic acid to observe the effect of chlorogenic acid on adjuvant arthritis model rats with left and right foot plantar width,serum tumor necrosis factor(TNF - alpha),interleukin 2(IL - 2),the a-mount of circulating immune complex(CIC),superoxide dismutase(SOD),glutathione peroxidase(GSH - Px)and malon-dialdehyde(MDA)content. Results The results demonstrated that chlorogenic acid(25 mg·kg - 1 and 100 mg·kg - 1 ) can inhibit rat ear swelling caused by dimethylbenzene and foot swelling caused by freund's adjuvant. Chlorogenic acid in medium dose group(50 mg·kg - 1 )and high dose group(100 mg·kg - 1 )could reduce the content of increase SOD and GSH - Px level,compare the difference was statistically significant(P ﹤ 0. 05). Conclusion The results demonstrated that chlorogenic acid had anti - inflammatory effect on adjuvant arthritis(AA)and by reducing the TNF - α,IL - 2,CIC and MDA content and increasing antioxidant capacity.