HPLC-ELSD法同时测定护肝片中猪去氧胆酸和鹅去氧胆酸的含量

摘要

Objective To establish an HPLC-ELSD method for the simultaneous determination of hyodeoxycholic acid and chenodeoxycholic acid in Hugan Tablets. Methods The chromatography condition were as follows:the analytical column was Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm)column,the mobile phase was methanol-0.2% formic acid so-lution(80:20),the flow rate was 1.0 mL·min-1,the column temperature was 30 ℃,the carrier gas flow rate of ELSD de-tector was 2.0 L·min-1 ,the drift tube temperature was 60 ℃,and the carrier gas was nitrogen gas. Results The linear ranges of hyodeoxycholic acid and chenodeoxycholic acid were 0. 521~10. 42 μg( r=0. 998 9)and 0. 266~5. 32 μg( r=0.999 4),with their average recoveries were 97.41%( RSD=1.30%,n=6)and 96.09%( RSD=2.11%,n=6),respectively. Conclusion The method was accurate,simple,sensitive,and it can be used for the quality control of Hugan Tablets.%目的：建立高效液相-蒸发光散射检测（ HPLC-ELSD）法同时测定护肝片中猪去氧胆酸和鹅去氧胆酸含量的方法。方法采用Agilent Zorbax SB-C18（4.6 mm×250 mm，5μm）色谱柱，流动相为甲醇-0.2%冰醋酸溶液（80：20），流速为1.0 mL·min-1，色谱柱温为30℃；蒸发光检测器载气为氮气，流速为2.0 L·min-1，漂移管温度为60℃。结果猪去氧胆酸在0.521~10.42μg范围内呈现良好的线性关系（ r＝0.9989），平均加样回收率为97.41%， RSD为1.30%（n＝6）；鹅去氧胆酸在0.266~5.32μg范围内呈现良好的线性关系（r＝0.9994），平均加样回收率为96.09%，RSD为2.11%（ n＝6）。结论该方法准确、简便、灵敏度高，可用于护肝片中猪胆粉的质量控制。