三七总皂苷对兔BMSCs的成骨分化及TGF-β1表达的影响

摘要

目的：观察三七总皂苷（panax notoginseng saponins，PNS）对兔骨髓间充质干细胞(bone marrow stromal cells， BMSCs)成骨分化及TGF-β1在基因水平表达变化的影响。方法：体外分离培养兔BMSCs，观察不同浓度PNS（50、100、200 mg/L）对兔BMSCs的影响，采用MTT法检测细胞增殖，茜素红染色观察钙结节形成，qRT-PCR检测TGF-β1表达水平。结果：各浓度PNS培养下的细胞增殖无明显差异（P＞0.05）；100、200 mg/L PNS组的钙结节数量和TGF-β1的表达水平均高于阴性组（P＜0.05）。结论：PNS可促进兔BMSCs的成骨分化并刺激其分泌TGF-β1，但无明显促细胞增殖作用。%Objective: To observe the osteoinduction effect of panax notoginseng saponins (PNS) on rabbit bone marrow mesenchymal stem cells (BMMSCs), and the expression level change of TGF-β1. Methods: Rabbit BMMSCs were isolated and cultured in vitro. To observe the influence of different concentration of PNS (50、100、200 mg/L) on rabbit BMMSCs, several methods were performed, including cell culture technique, MTT colorimetric assay, Dahl McGec Russell's Alizarin red staining for calcium and qRT-PCR explore TGF-β1 expression level. Results: There was no significant difference of proliferation capacity of BMMSCs between various concentration of PNS (P>0.05). The calcium nodule and TGF-β1 expression level in 100、200 mg/L PNS groups were significantly higher than negative group (P<0.05). Conclusion: PNS can promote the osteogenic differentiation and secretion of TGF-β1 of rabbit BMMSCs, but PNS is not effective enough for mediation of BMMSCs proliferation.