银杏叶提取物对肉仔鸡十二指肠黏膜免疫的调节作用

摘要

【Objective】 An experiment was conducted to investigate the modulatory effects of Ginkgo biloba extract（EGB） on duodenum mucosal immunity of broilers.【Method】 Three hundred and sixty one-day-old healthy Arbor Acres female broilers were divided into five groups,each with six replicates.The first group was the control group and fed a basal diet,while the other four groups were fed the same basal diet supplemented with 0.009% aureomycin or 0.15%,0.20%,0.25% EGB.The experiment lasted 42 days.The contents of immunoglobulin,the numbers of mucosal immunity-associated cells and the level of interleukin-2（IL-2） in duodenum mucosa were determined.【Result】 Adding 0.20% EGB or 0.25% EGB to diet significantly increased the contents of IgA,IgG and IgM in duodenum mucosa of 42-day-old broilers（P0.05） compared with the control and the aureomycin.The numbers of intraepithelial lymphocytes,mast cells and goblet cells and the level of IL-2 in duodenum mucosa were also significantly elevated in 42-day-old broilers fed diets supplemented with 0.20% EGB or 0.25% EGB（P0.05）.【Conclusion】 Adding 0.20% EGB or 0.25% EGB to diets of broilers could enhance duodenum mucosal immunity of broilers.%【目的】研究银杏叶提取物（EGB）对肉仔鸡十二指肠黏膜的免疫调节作用。【方法】将360只1日龄健康AA肉用母雏鸡随机分成5组,每组6个重复,其中1组为对照组,饲喂基础日粮;其余4组分别饲喂添加质量分数0.009%金霉素及0.15%,0.20%和0.25%EGB的日粮,试验期为42d。分别于21和42d取样,测定各组肉仔鸡十二指肠黏膜免疫球蛋白含量、黏膜免疫相关细胞数量和白细胞介素-2（IL-2）含量。【结果】日粮中添加质量分数0.20%,0.25%EGB可显著提高42日龄肉仔鸡十二指肠黏膜IgA、IgG、IgM含量（P〈0.05）,提高十二指肠上皮内淋巴细胞、肥大细胞、杯状细胞数量和IL-2含量（P〈0.05）,并且作用效果优于金霉素组（P〈0.05）。【结论】日粮中添加质量分数0.20%或0.25%EGB,可增强肉仔鸡十二指肠黏膜免疫功能。