多能干细胞来源的肝细胞诱导分化及其应用研究进展

摘要

胚胎干细胞(embryonic stem cells,ESCs)和诱导多能干细胞(induced pluripotent stem cells,iPSCs)是一类具有多能性和自我更新能力的多能干细胞,这些细胞具有无限增殖能力,并有潜能分化为机体三胚层所有类型的细胞.多能干细胞来源的肝细胞能够作为肝细胞的替代物应用于体外药物筛选和肝脏疾病的治疗.文章对多能干细胞向肝细胞的诱导研究、多能性干细胞来源肝细胞的鉴定方案研究及其在药物筛选和疾病治疗方面的研究进行了综述分析,并对未来的研究方向进行了总结与展望.%Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are pluripotent stem cells with the characteristics of self-renewal and pluripotency.They have the ability of infinite proliferation and forming all types of cells in the three germ layers.The pluripotent stem cells derived hepatocyte-like cells could serve as alternative to liver cells applied in early pharmaceutical screening and treatment of liver diseases.In this paper,we review the strategies of producing hepatocyte-like cells from pluripotent stem cells,the assessment of pluripotent stem cells derived hepatocyte-like cells,and the application of pluripotent stem cells derived hepatocyte-like cells in pharmaceutical screening and treatment of liver diseases.Future research directions are also prospected.