杂交兰组培苗与内生真菌共生培养体系的构建

摘要

为了筛选出对杂交兰组培苗生长有益的菌株，采用4种内生真菌（被孢霉属Mortierella sp．），通过固体菌剂和液体菌剂2种接种方式，建立杂交兰组培苗与真菌的共生培养体系。共生培养150 d后，运用石蜡切片法观察到菌丝、菌丝结等典型的菌根结构。结合重分离真菌形态观察和分子鉴定，确定重分离菌株均为原接种菌株。结果表明：液体菌剂接种方式相比固体菌剂，对杂交兰组培苗生长的促进作用显著；菌株FC5和FC9液体菌剂对杂交兰组培苗的生长促进作用显著；FC17液体菌剂对杂交兰组培苗根部生长促进效果显著。%To screen out beneficial fungi strains to Cymbidiumh ybridum (C.hybridum‘Princess Nobuko’×C.goeringii‘Huan-gshuixian’ ) plantlets growth, using solid inoculation and liquid inoculation, we established mycorrhizal symbiosis system between four kinds of endophytic fungi ( Morit erell a sp).and Cymbidium hybridum plantlets.After 150-day symbiotic cul-ture, typical mycorrhizal structures such as hyphae and pelotons were founded by permanent paraffin-cut section method. Considering morphological observation and molecular identification, it was confirmed that fungi isolated from C.hybridum plantlets roots were the same as origin fungi.The liquid inoculum treatment was better than solid inoculum treatment. Strains FC5 and FC9 liquid inoculum treatments had obvious promoting effect on the growth of C.hybird um plantlets.Strain FC17 liquid inoculum treatments significantly promoted the growth of C.hybridum plantlet roots.