高效液相色谱法测定健胃咀嚼片中橙皮苷的含量

摘要

目的：建立高效液相色谱法测定健胃咀嚼片中主要的功效成分陈皮中橙皮苷的含量。方法：WatersC18柱（XBridgeTMC18；5μm；4.6mm×250mm）；以甲醇∶冰醋酸∶水（34∶4∶62）为流动相；检测波长283nm。结果：橙皮苷在48.45～339.51ng范围内呈良好的线性关系，r=0.9998，平均加样回收率为105.11%，RSD为1.9%（n=9）。结论：本方法操作简便，结果准确，重现性好，可用于健胃咀嚼片的质量控制。%Objietive: To determine the content of JianWei chewable Tablets of dried tangerine peel,Methods:C18 column and methanol-acetic acid-water (34∶4∶62)as mobile phase,detection wavelength283nm.Results:The linear rang was 48.45~339.51ng,r=0.9998.The average recovery rat was 105.11%,RSD=1.9%(n=9). Conclusions:This method is simplify the operation,accurate,highly reproducible and can be used for the quality control of JianWei chewable Tablets.