参芪扶正注射液对急性肺损伤大鼠肺组织热休克蛋白-70表达的影响

摘要

Objective To explore the expression of heat shock protein 70 (HSP-70)in lung tis-sue of rats with endotoxin-induced acute lung injury (ALl)and the protection mechanism of Shenqi Fuzheng Injection on it.Methods A total of 30 SD rats were randomly divided into three groups:ALI group,blank control group,Shenqi Fuzheng Injection group.After 2 hours of the experiment beginning to killed all rats.to determined the blood oxygen saturation and lung coefficient of each rats,and to ob-served the histopathological changes by HE staining and detected the expression of HSP-70 in lung tissue by western blotting.Results The lung coefficient increased significantly,blood oxygen satura-tion decreased obviously in ALI group compared to Shenqi Fuzheng Injection group,the expression of HSP-70 in lung tissues decreased in ALI group obviously than Shenqi Fuzheng Injection group .The lung tissues saw a lot of multi-core leukocyte infiltration,wider alveolar septum,alveolar hemorrhage and alveolar integrity in ALI group rats;the damage degree of rats in Shenqi Fuzheng Injection group were ligher than the ALI group,Pulmonary interstitial had light to moderate edema,also leukocyte in-filtration was lighter than ALI group.Conclusion Shenqi fuzheng injection to the ALI rats lung tissue have good protection effect,may be associated with induction of HSP - 70 expression.%①目的探讨内毒素致急性肺损伤（ALI）大鼠肺组织中热休克蛋白-70（HSP-70）的表达以及参芪扶正注射液对 ALI 的保护作用机制。②方法30只 SD 大鼠随机分为空白对照组、ALI 组、参芪扶正组。ALI 组腹腔注射内毒素，参芪扶正组腹腔注射内毒素、参芪扶正注射液，空白对照组不作任何处理。均于实验开始2小时后处死，测定各组大鼠血氧饱和度、肺系数，用 HE 染色观察组织病理学改变，蛋白印迹方法检测肺组织中 HSP-70的表达。③结果 ALI 组较参芪扶正组肺系数明显增加、血氧分压明显下降、肺组织中 HSP-70的表达明显降低（P 均＜0．05）。ALI 组大鼠肺组织光镜下见大量多核白细胞浸润、肺泡隔增宽、肺泡内出血以及肺泡完整性破坏；参芪扶正组大鼠肺组织损伤程度较 ALI组减轻，肺间质有轻至中度水肿，白细胞浸润也较 ALI 组轻。④结论参芪扶正注射液对 ALI 大鼠的肺组织起保护作用，可能与诱导 HSP-70表达有关。