THE EXPRESSION OF HEAT SHOCK PROTEIN 70 IN RAT BRAIN AFTER +Gz EXPOSURE

摘要

Purpose: To investigate the effect of +Gz exposure on the expression and distribution of heat shock protein 70 (HSP70) in rat brain. Methods: One hundred rats were randomly divided into control group, +2Gz, +6Gz and +10Gz exposures groups. The +Gz group rats were exposed to +2Gz, +4Gz, +6Gz and +10Gz for 3 minute respectively. The expression of HSP70 in rat brain was measured by immunohistochemistry and West blot methods after +Gz exposure. Results: There was no HSP70 expression in the brains of control rats. In +2, +4, and +6Gz groups, HSP70 was obviously expressed in cortex, hippocampus and pyriform cortex 6h after exposures, and strongly expressed 1d after exposure, and then had a tendency to decrease 2d after exposure, and returned to control level 6d after exposure. The expression of HSP70 after +6Gz exposure was the strongest in all +Gz groups. In +10Gz group, HSP70 protein was only weakly expressed in pyriform cortex after exposure. Conclusions: +Gz exposures may cause time-dependent HSP70 expression in rat brain.