热休克蛋白60在激活的小胶质细胞中的表达及基因调控

摘要

Objective To explore the expression of HSP60 in activated microglia cell and its gene regulation. Methods Microgla cells were treated with LPS and cells were collected for western - blot. RNA were extracted from which for real - time PCR analyzing. Results In activated microgla cells, both HSP60 protein and mRNA levels significantly increased and the expression of its transcription factor HSF - 1 increased. Conclusion In activated microglia cells, HSP60 and its transcription factor HSF -1 highly expressed to participate the stress response.%目的 研究细菌脂多糖(LPS)激活的小胶质细胞中热休克蛋白60(HSP60)的表达情况及其基因调控.方法 分离SD大鼠的小胶质细胞,经LPS激活后,应用Westem- Blot方法检测细胞中HSP60蛋白的表达,real - time PCR法检测细胞中HSP60 mRNA的量,并进一步检测热休克因子1(HSF-1)表达的变化.结果 在激活的小胶质细胞中,HSP60的蛋白及mRNA水平均湿著升高,其转录因子HSF -1的水平也相应增高.结论 在激活的小胶质细胞中,HSF -1蛋白表达显著提高,HSP60mRNA及蛋白合成增强,参与细胞的应激反应.