基于多因子降维法的肺结核易感基因-基因交互作用研究

摘要

Objective To analyze gene -gene interactions in tuberculosis using Multifactor Dimensionality Reduction (MDR) model. Methods The variant genotypes of MCP - 1、 CCR2、 VDR、 3 UTR、 D543N、 INT4 and MBL- G54A were identified by PCR- RFLP. The gene -gene interactions were analyzed with the MDR model. Results The interaction between VDR and CCR2 gave the best MDR model with statistical significance. The model Testing Balance Accuracy was 0. 6248. The Cross - Validation consistency was 10/10.Conclusion The gene -gene interaction with related to the genetic polymorphism of VDR and CCR2 may increase the risk of tuberculosis.%目的 分析宁夏银川地区肺结核易感基因间的交互作用.方法 结合前期病例对照研究结果(PCRRFLP法检测肺结核易感基因MCP-1、CCR2、VDR、NRAMP1-3'UTR、NRAMP1D543N、NRAMP1INT4及MBLG54A分型,银川地区肺结核病例271例,健康对照299例),运用MDR法分析肺结核易感基因-基因交互作用模型.结果 有统计学意义的最佳交互模型包含VDR基因和CCR2基因,该模型的检验样本准确度为0.6248,交叉验证一致性的结果为10/10.结论 VDR基因和CCR2基因可能协同增强了宿主对结核杆菌的易感性,从而增加了宿主肺结核病的患病风险.