羊水、脐带和骨髓来源间充质干细胞对淋巴细胞增殖的抑制效果比较

摘要

Objective To compare the inhibitory effect of amniotic fluid-derived mesenchymal stem cells ( AF-MSC) , umbilical cord blood mesenchymal stem cells ( UC-MSC ) and bone marrow Mesenchymal stem cells ( BM-MSC) on phytohemagglutinin ( PHA) -induced lymphocyte proliferation.Methods Human mesenchymal stem cells from the second trimester amniotic fluid , umbilical cord and normal adult bone mar-row, flow cytometry were extracted and detected the surface molecules of MSC.Induce the MSCs differentiate into osteogenic like and adipogenic like cells in virto.AF-MSC, UC-MSC and BM-MSC were added to PHA-in-duced lymphocyte proliferation system at different ratio , compared inhibitory effect of three different sources-de-rived mesenchymal stem cells on lymphocyte proliferation.Results Mesenchymal stem cell-specific surface molecules CD29, CD70 and CD90 were expressed by isolated AF-MSC, UC-MSC and BM-MSC, respectively.However, hematopoietic stem cell surface molecule CD 45 was not expressed.In vitro cultures of AF-MSC, UC-MSC and BM-MSC on lymphocyte proliferation inhibition , when lymphocytes: mesenchymal stem cells 1: 1, 1: 10, 1: 50, 1: 100, the right lymphocyte proliferation inhibition rate AF-MSC strongest, BM-MSC wea-kest.Whenever the of ratio of lymphocytes and mesenchymal stem cells are 1: 1, 1: 10, 1: 50, 1: 100, the lymphocyte proliferation inhibition of AF-MSC is strongest , and BM-MSC is weakest.Conclusion AF-MSC, UC-MSC and BM-MSC can inhibit PHA-induced lymphocyte proliferation , in which the AF-MSC is the strongest and BM-MSC is the weakest.Further study indicates that the effect was associated with the number of ratio of MSC to LC.%目的：比较羊水来源间充质干细胞（ AF-MSC）、脐带来源间充质干细胞（ UC-MSC）和骨髓来源间充质干细胞（ BM-MSC）对植物血凝素诱导的淋巴细胞增殖的抑制作用。方法从妊娠中期羊水、新生儿脱落脐带、正常成人骨髓中分离获取原代AF-MSC、 UC-MSC和 BM-MSC。观察形态并采用流式细胞术检测表面分子。取传代培养的第3代的AF-MSC、 UC-MSC和 BM-MSC体外诱导培养后，观察体外诱导成骨成脂多向分化情况。将不同细胞浓度的AF-MSC， UC-MSC和 BM-MSC分别加入到植物血凝素诱导的外周血淋巴细胞增殖体系中，比较3种不同来源的MSC对淋巴细胞增殖的调控作用。结果分离培养的AF-MSC， UC-MSC和BM-MSC分别表达MSC特异性表面分子CD29、CD70、 CD90，不表达造血干细胞表面分子CD45。体外传代培养的AF-MSC、 UC-MSC和BM-MSC对淋巴细胞增殖的抑制中，当淋巴细胞∶MSC为1∶1、1∶10、1∶50、1∶100时，对淋巴细胞增殖的抑制率AF-MSC最强， BM-MSC最弱。结论 AF-MSC、 UC-MSC和BM-MSC均具有抑制植物血凝素诱导淋巴细胞增殖的作用，其中以AF-MSC抑制效果最强， BM-MSC最弱，其抑制效果与MSC数量有关。