miR-92b对神经胶质瘤细胞U251增殖及迁移侵袭的影响

摘要

目的 探究miR-92b对神经胶质瘤细胞U251增殖及迁移侵袭的影响以及其可能机制.方法 利用miRNA芯片筛选出在神经胶质瘤细胞U251和人脑正常胶质细胞HEB中差异表达的miRNA;化学合成法制备miR-92b抑制剂,转染后用real-time PCR技术验证表达变化;CCK-8实验检测转染后细胞的增殖能力;划痕实验和Transewll实验检测转染后细胞的迁移和侵袭能力;Western blot检测细胞中胰岛素样生长因子结合蛋白3(IGFBP-3)、β-catenin和E-cadherin的表达;荧光酶素实验验证IGFBP-3是否为miR-92b的靶基因.结果 基因芯片结果显示miR-92b在神经胶质瘤细胞中表达水平高于人脑正常胶质细胞(P<0.05);CCK-8实验结果显示转染miR-92b抑制剂后,U251细胞增殖能力降低(P<0.05);划痕实验和Transwell实验结果显示转染miR-92b抑制剂后,U251细胞迁移和侵袭能力降低(P<0.05);Western blot结果显示抑制miR-92b后,IGFBP-3和E-cadherin蛋白表达增加(P<0.05),β-catenin表达减少(P<0.05);荧光素酶实验结果显示,miR-92b能直接靶向调控IGFBP-3.结论 miR-92b在神经胶质瘤中表达显著增加,其可能通过抑制IGFBP-3蛋白表达,从而促进肿瘤细胞的增殖及迁移侵袭.%Objective To investigate the effect of miR-92b on proliferation, migration and inva-sion of U251 cells and its possible mechanism. Methods The differential expression of miRNA in glioma cell line U251 and human normal glial cell line HEB was screened by miRNA chip. miR-92b inhibitor was synthe-sized by chemical synthesis, the expression changes were verified by real-time PCR after transfection. Cell pro-liferation ability was detected by CCK-8 assay after transfection. Wound healing assay and Transwell assay were used to detected cell migration and invasion ability after transfection. Western blot was performed to detected U251 cell proliferation and migration related protein β-catenin, E-cadherin and IGFBP 3. Luciferase assay was detected to confirm wheather IGFBP-3 was the target gene of miR-92b. Results miRNA chip results showed that the level of miR-92b in U251 cell was significantly higher than that of HEB. Inhibited miR-92b significant-ly decreased U251 cell proliferation ability, migration and invasion (P<0.05). Western blot results showed the expression of β-catenin was decreased (P<0.05) , and the expression of E-cadherin was increased (P<0.05) after transfection miR-92b inhibitor. Luciferase assay showed that miR-92b could directly regulate IGFBP-3. Conclusions The expression of miR-92b in U251 cell was increased significantly. It may inhibit the U251 cell proliferation and migration through up-regulation the expression of IGFBP-3 protein.