人参皂苷 Rb 1在体外肠道菌群模型中的代谢研究

摘要

OBJECTIVE Research on the gradual deglycosylated metabolism of ginsenoside Rb1 by intestinal flora under aer-obic and anaerobic conditions in vitro .METHODS The changes of Rb1 and its metabolites were detected by means of UPLC-MS/MS incubated by intestinal bacteria under aerobic and anaerobic conditions in vitro after 0,2,4,8,12,24,48 h and 72 h in vitro .RESULTS It was found that Rb1 was converted into three metabolites ginsenoside Rd,F2 and C-K by rat intestinal bac-teria in vitro through gradual removal of glycosylation while Rd→F2 was the rate-limiting step.The metabolic pathway of Rb1 by intestinal bacteria was Rb1 →Rd→F2 →C-K.CONCLUSION The deglycosylated metabolites of Rb1 under aerobic and anae-robic conditions have no differences,but the metabolic rate under aerobic conditions is faster.%目的：研究人参皂苷 Rb1在厌氧和有氧条件下经肠道菌群作用下逐级脱糖基的代谢差异。方法利用 UPLC-MS／MS研究人参皂苷 Rb1在厌氧和有氧条件下经肠菌液的作用下，分别温孵0、2、4、8、12、24、48、72 h 后，人参皂苷 Rb1及其脱糖基代谢物的变化情况。结果体外温孵实验中，人参皂苷 Rb1在厌氧和有氧条件下经大鼠肠菌液作用下脱糖基生成人参皂苷 Rd、F2、C-K，其中 Rd→F2是 Rb1在肠菌液的作用下脱糖基代谢的限速步骤。人参皂苷 Rb1在大鼠肠道菌群作用下代谢途径为Rb1→Rd→F2→C-K。结论人参皂苷 Rb1在有氧和厌氧条件下脱糖基代谢产物没有差异，有氧代谢速率快于厌氧代谢。