透明质酸修饰的葛根素 PEG-PLGA 纳米粒的处方工艺优化及其体外评价

摘要

ABSTRACT:OBJECTIVE With PEG-PLGA as the carrier,and the preparation of hyaluronic acid modified kudzu root ele-ment PEG-PLGA nanoparticles by nanoprecipitation method,to optimize the preparation prescription,and preliminary evalua-tion of its properties in vitro.METHODS With PEG PLGA as the carrier,Hyaluronic acid as surface modification agent, preparation of hyaluronic acid modified kudzu root element PEG-PLGA HA∕Pue-NPs by nanoprecipitation method:design and optimize the prescription by Orthogonal experimental,characterize its in vitro properties,examine the in vitro drug release be-havior of HA∕Pue-NPs.RESULTS Prepared drug-loading nanoparticles has the spherical appearance.The average particle size and Zeta potential are(88.9±2.2 )nm、(-21.9± 0.54)mV respectively,drug loadings and the coating rate are 6.75%, 78.52%.In vitro drug release test shows that the drug-loading nanoparticles release slowly,the accumulative release rate is 65.8% within 24h.CONCLUSION The particle size of HA∕Pue-NPs is consistent,good in vitro properties and certain slow-release characterized.%目的：以乳酸-羟基乙酸共聚物(PEG-PLGA)为载体,优化纳米沉淀法制备透明质酸修饰的葛根素 PEG-PLGA 纳米粒(HA/Pue-NPs),并对其体外性质进行初步评价。方法以 PEG-PLGA 为载体材料,透明质酸为表面修饰剂,采用纳米沉淀法制备了透明质酸修饰的 HA/Pue-NPs；应用正交实验设计优化处方,对其体外性质进行表征；并采用体外释药行为评价透明质酸修饰 HA/Pue-NPs。结果制备出的载药纳米粒外观呈球形,平均粒径、Zeta 电位分别为(88.9±2.2)nm、(-21.9±0.54)mV,载药量及包封率分别为6.75%、78.52%。体外释药试验表明,载药纳米粒释药缓慢,24 h 的累计释放率为65.8%。结论透明质酸修饰的葛根素 PEG-PLGA 纳米粒粒径大小均一,体外性质良好且具有一定的缓释特性。