BMSCs移植对自发性AD鼠神经元及突触变化影响机制的研究

摘要

Objective:To explore the effects of bone marrow mesenchymal stem cells transplantation on plasticity in spontaneous aged dementia mice degeneration of neurons and synapses.Methods:The sterile healthy bone marrow stromal stem cells were cultured and identified , rats were divided into normal control group , DMEM control group and transplantation of bone marrow stromal stem cells transplantation group.Bone marrow stromal stem cells transplantation group as intravenous transfusion in the PKH 26 tag,with RT-PCR method, morphological detection , electron microscope observation of bone marrow stromal cells transplantation on senile dementia rat neuronal degeneration and outstanding plasticity.Results:Through the RT-PCR method, morphological detection , electron micro-scope , bone marrow stromal stem cell number cell transplantation group , has been added;at the same time , bone marrow stromal stem cells secrete neurotrophic factor , the in vivo micro environment is improved , leading to the formation of micro environment conducive to repair and the neurons were offered the corresponding protections against necnosis .the number of which in the control group was signifi-cantly higher than that of DMEM transplantation , close to the normal control group , neuronal survival cell number.The micro environ-ment is improved, is conducive to the establishment of synaptic connection with surrounding cells effectively .Conclusion:Bone marrow stem cell transplantation has protective effects on spontaneous aged dementia rat neuron degeneration and outstanding plasticity .%目的：探索骨髓基质干细胞移植对自发性老年痴呆鼠神经元变性及突触可塑性变化的影响。方法：无菌将 Wistar大鼠骨髓基质干细胞进行培养及鉴定，实验分为正常对照组、DMEM移植对照组、骨髓基质干细胞移植组。骨髓基质干细胞移植组在PKH26标记后进行尾静脉回输，再用RT－PCR 方法、组织形态检测、电镜观察等方法进行研究骨髓基质干细胞移植对老年痴呆鼠神经元变性及突触可塑性的变化。结果：通过RT－PCR 方法、组织形态检测、电镜观察，骨髓基质干细胞移植组的神经元细胞数，其得到了补充；同时骨髓基质干细胞分泌的神经营养因子，体内微环境得到改善，进而形成有利于修复的微环境，未坏死的神经元得以相应的保护。其明显高于DMEM移植对照组，接近正常对照组神经元存活细胞数。改善后的微环境，有利于其与周围细胞建立有效的突触联系。结论：骨髓基质干细胞移植对自发性老年痴呆鼠神经元变性及突触可塑性具有保护作用。