Objective To analyze the epidemiological characteristics of human bocavirus (HBoV) in children with acute respiratory tract infection in Suzhou. Methods 6108 nasopharyngeal aspirate specimens were collected from hospitalized children with acute respiratory tract infection. HBoV was detected by using real time fluorescence PCR. Mycoplasma pneumonia (MP) was detected by using real time fluorescence quantitative PCR. RSV, IVA, IVB, PIV1~3 and ADV were detected by using direct immunofluorescence assay. Results The total incidence rate of HBoV infection was 6.19%(378/6108), in which 73 cases (19.31%,73/378) were mixed with other pathogens. RSV, PIV-3 and MP were the most preferred pathogens mixed with HBoV infection, and their incidence rate was 64.38%(47/73),16.44%(12/73) and 12.33%(9/73), respectively. Positive rate of HBoV infection in the summer, autumn, winter and spring were 9.06%,11.35%,3.52% and 2.37%, respectively. There was a significant difference between summer and spring, winter, also, between autumn and spring, winter (P<0.01 for all). On the population distribution, the positive incidence rate of HBoV in different age group were 11.14% for 7~12 months old,10.40% for 1~3years old,2.83% for 0~6 months old,4.29% for 3~5 years old,3.11% for 5~6 years old and 1.97% for over 6 years old, respectively. The positive rate of HBoV was higher in 7 months to 3 year old children than other age groups (χ2=144.26, P<0.01). Conclusion HBoV is one of the main pathogens in children with acute respiratory infection in Suzhou area, and it is occurred throughout the year. There were high incidences of HBoV infection in summer and autumn. Mixed infection with other pathogens had a high proportion for HBoV, and RSV was its most preferred partner. The children from 7 months to 3 years old were the most susceptible population for HBoV infection.% 目的分析人博卡病毒(human bocavirus,HBoV)在苏州地区儿童急性呼吸道感染患儿中的流行特征.方法收集6108例急性呼吸道感染的住院儿童鼻咽分泌物标本,实时荧光 PCR 法检测 HBoV,同时采用实时荧光定量 PCR 检测肺炎支原体(MP),直接免疫荧光法检测呼吸道合胞病毒(RSV)、流感病毒 A 型、B 型(IVA,IVB)、副流感病毒1~3型(PIV1~3)和腺病毒(ADV).结果共检出 HBoV 感染378例(6.19%,378/6108),其中 HBoV 与其他病原体混合感染73例(19.31%,73/378).与 HBoV 混合感染的前三位病原体分别为 RSV (64.38%,47/73),PIV-3(16.44%,12/73),MP(12.33%,9/73).HBoV 感染阳性率在夏秋季分别为9.06%和11.35%,而在冬春季3.52%和2.37%,夏秋和冬春各季之间阳性率存在显著性差异(均P<0.01).HBoV 感染在7~12月和1~3岁儿童中的阳性率分别为11.14%和10.40%,而在0~6月组、3~5岁组、5~6岁组和≥6岁组的阳性率分别为2.83%、4.29%、3.11%和1.97%,7月~3岁患儿与其它年龄组间 HBoV 阳性率存在显著性差异(χ2=144.26,P<0.01).结论人博卡病毒是苏州地区儿童急性呼吸道感染的主要病原之一,苏州全年均存在 HBoV 感染,以夏秋季高发.HBoV 易与其它病原混合感染,尤其是 RSV.HBoV 感染在7月~3岁儿童中高发.
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